RCSB PDB - 1SXJ: Crystal Structure of the Eukaryotic Clamp
Mar 30, 2004 · Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA).
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PDB-1sxj: Crystal Structure of the Eukaryotic Clamp Loader
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RCSB PDB - 1SXJ: Crystal Structure of the Eukaryotic Clamp
Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of ATPases. We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA.
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Dynamics of Open DNA Sliding Clamps
May 05, 2016 · Bowman GD, O'Donnell M, Kuriyan J. Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature. 2004;429(6993):724–30. Epub 2004/06/18. pmid:15201901 . View Article PubMed/NCBI Google Scholar 20. Kelch BA, Makino DL, O'Donnell M, Kuriyan J.
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How a DNA polymerase clamp loader opens a sliding …
Abstract. The eukaryotic RFC clamp loader couples the energy of ATP hydrolysis to open and close the circular PCNA sliding clamp onto primed sites for use by DNA polymerases and repair factors. Structural studies reveal clamp loaders to be heteropentamers.
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Structural insight into recruitment of translesion DNA
Jan 14, 2009 · Our structural analysis of Dpo4 in the Dpo4–PCNA complex reveals a third major conformation for this model Y-family polymerase, in addition to the apo- and DNA-bound Dpo4 structures. We have identified two intrinsically flexible hinge regions in Dpo4, based on its multiple conformations ( Ling et al ., 2001 ; Fiala et al ., 2007 ; Wong et al
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Structure of the human clamp loader reveals an
Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate …
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The RFC Clamp Loader: Structure and Function | SpringerLink
Jul 07, 2012 · Bowman GD, O'Donnell M, Kuriyan J (2004) Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature 429:724–730 PubMed CrossRef Google Scholar Bowman GD, Goedken ER, Kazmirski SL, O'Donnell M, Kuriyan J (2005) …
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PDB-1sxj: Crystal Structure of the Eukaryotic Clamp Loader
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DNA's Grand Prix | Science Beat
Aug 27, 2004 · "Structural analysis of a eukaryotic sliding DNA clamp–clamp loader complex," by Gregory D. Bowman, Mike O'Donnell, and John Kuriyan, appears in the 17 June 2004 issue of Nature. More about research in the Kuriyan Lab : Top
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Crystal Structure of the Eukaryotic Origin Recognition Complex
Mar 19, 2015 · Crystal structure of the ORC hexamer. Sequence analyses had indicated that the Orc1-5 subunits would share a domain architecture similar to that of archaeal Orc proteins, with AAA+-type ATPase folds fused to at least one C-terminal winged-helix (WH) DNA-binding domain (Fig. 1a) 7,8.For its part, the Orc6 C-terminus has been reported to bind to ORC1-5 through a domain insertion in Orc3, …
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Kuriyan Lab - Publications
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex Gregory D. Bowman, Mike O'Donnell and John Kuriyan Nature 2004 Jun 17;429(6993):724-30
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How a DNA polymerase clamp loader opens a sliding clamp.
A) The fully ATP-bound conformation of the open clamp:clamp loader:DNA complex from form II crystals. The clamp loader holds the clamp such that there is a gap between subunits I and III of the clamp. B) Conformational changes in the clamp:clamp loader:DNA complex from ATP hydrolysis at the B subunit as seen in form III crystals.
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Structure of the human clamp loader reveals an
Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate …
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Structural and Thermodynamic Analysis of Human PCNA with
Human Proliferating Cellular Nuclear Antigen (hPCNA), a member of the sliding clamp family of proteins, makes specific protein-protein interactions with DNA replication and repair proteins through a small peptide motif termed the PCNA-interacting protein, or PIP-box. We solved the structure of hPCNA bound to PIP-box-containing peptides from the p66 subunit of the human replicative DNA
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The loader of the rings | Nature
Jun 17, 2004 · The loader of the rings. Among the numerous molecular machines involved in the process of DNA replication are the ring-shaped sliding clamp and …
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DNA Sliding Clamps: Just the Right Twist to Load onto DNA
Dec 24, 2005 · Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature. 2004; 429 : 724-730 View in Article
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Structural analysis of a eukaryotic sliding DNA clamp
These load ring-shaped sliding clamps onto DNA to keep polymerase attached during replication and contain one δ, three γ, and one δ' AAA+ subunits semi-circularly arranged in the order δ-γ1
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[PDF] DNA polymerase clamp loaders and DNA recognition
Feb 07, 2005 · The crystal structures of clamp loader complexes are reviewed and the insights they have provided into the mechanism of the clamp loading process are reviewed. Clamp loaders are heteropentameric ATPase assemblies that load sliding clamps onto …
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The RFC Clamp Loader: Structure and Function
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